Fabrication and analysis of nano-hydroxyapatite [Ca10(PO4)6(OH)2] composites with collagen derived from eggshells through freeze-drying

Maming, Maming; Mubakira, Fadliah; Raya, Indah; Musa, Bulkis; Anshar, Andi Muhammad; Mayasari, Erna; Hala, Yusafir; Kasim, Syaharuddin; L., Andi Ilham; Alam, Gemini; Usman, Andi Nilawati; Hasmawati, Hasmawati; Hasri, Hasri; Usman, Andriani; Irfandi, Rizal

doi:10.48309/ecc.2023.418678.1689

Document Type : Original Research Article

Authors

¹ Department of Chemistry, Faculty of Mathematics and Natural Science, Hasanuddin University, Makassar 90245, Indonesia

² Universitas Hasanuddin

³ Faculty of Medicine, Hasanuddin University, Makassar, Makassar 90245, Indonesia

⁴ dMidwifery Study Program, Hasanuddin University, Makassar 90245, Indonesia

⁵ Department of Matematics, Faculty of Mathematics and Natural Sciences, Hasanuddin University, Makassar 90245, Indonesia

⁶ Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Negeri Makassar, Makassar 90244, Indonesia

⁷ Department of Biology Education, Faculty of Teacher Training and Education, Universitas Puangrimaggalatung, Sengkang 90915, Indonesia

10.48309/ecc.2023.418678.1689

Abstract

This study aims to know how to composite hydroxyapatite-collagen by freeze-drying method, and the characterization of hydroxyapatite-collagen composites using XRD, FTIR, and SEM. Hydroxyapatite nanocrystals are synthesized using the precipitation method by reacting CaO and (NH₄)₂HPO₄ and the freeze-drying method in composite manufacturing by reacting hydroxyapatite nanocrystals with collagen. The results showed that hydroxyapatite nanocrystals can be composite with collagen using the freeze-drying method, and the results of characterization of XRD, FTIR, and SEM hydroxyapatite-collagen composites show the formation of chemical bonds between hydroxyapatite and collagen. The XRD spectrum of HAp-Collagen shows a peak at 2θ 10.45°, showing a physically formed composite. FTIR characterization shows the presence of absorption bands PO₄^3- and OH^- from HA, slight wavenumber shifts also occur in C-H, C-N, N-H, and C=O groups which are characteristic of collagen, this indicates that composites are formed physically. SEM characterization shows HAp is perfectly deposited into collagen molecules.

Graphical Abstract

Keywords

Main Subjects

Inorganic chemistry

References

[1] M. Chandran, K. Brind’Amour, S. Fujiwara, Y-C. Ha, H. Tang, J-S. Hwang, J. Tinker, J.A. Eisman , Prevalence of osteoporosis and incidence of related fractures in developed economies in the Asia Pacific Region: a systematic review, Osteoporos Int., 2023, 34, 1037–1053. [Crossref], [Google Scholar], [Publisher]

[2] M. Sivakumar, T.S. Sampath Kumar, K.L. Shanta, R.K. Panduranga. Development of hydroxyapatite derived from Indian coral, J. Biomater., 1996, 17, 1709-1714. [Crossref], [Google Scholar], [Publisher]

[3] A). E. Garetta, T. Fernandez, S. Borros, J. Esteve, C. Colominas, L. Kempf, Synthesis of biocompatible surfaces by different techniques, J. Mat. Res. Soc. Symp. Proc, 2002, 724, N8.11.1-N8.11.6. [Crossref], [Google Scholar], [Publisher] b). R. Kareem, N. Bulut, O. Kaygili, Hydroxyapatite biomaterials: a comprehensive review of their properties, structures, medical applications, and fabrication methods, J. Chem. Rev., 2024, 6, 1-26. [Crossref], [Pdf], [Publisher]

[4] a). C.D. Nascimento, Biomaterials applied to the bone healing process, Int. J. Morphology, 2007, 25, 839-846. [Google Scholar], [PDF] b). B. Khatri, A. Rajbhandari (Nyachhyon), Preparation, characterization and photocatalytic application of novel bismuth vanadate/hydroxyapatite composite, Adv. J. Chem. A., 2020, 789-799. [Crossref], [Google Scholar], [Publisher] c). A.A. Radhi, H. Aljudy, The impact of mixed NaOH and KOH mole fraction on mechanical performance of metakaolin based geopolymer material, J. Med. Chem. Sci., 2023, 6, 1120-1128. [Crossref], [Pdf], [Publisher] d) H.A. Ameer Radhi, M. Abdulaziz Ahmad, Biological test of porous geopolymer as a bone substitute, J. Med. Chem. Sci., 2023, 6, 710-719. [Crossref], [Google Scholar], [Publisher]

[5] S. Ahmed, M. Ahsan, Synthesis of Ca-hydroxyapatite bioceramic from egg shell and its characterization, Bangladesh J. Sci. Ind. Res., 2008, 43, 501-512. [Google Scholar], [Publisher]

[6] T.Q. Abd Alkareem, E.J. Waheed. Formation, characterization and antioxidant study of mixed ligand complexes derived from succinyl chloride, Chem. Methodol., 2022, 6, 914-928. [Crossref], [Pdf], [Publisher]

[7] A.E. Ahmed Tamer, W. Ling, Y. Manar, H. Maxwell, Biotechnological applications of eggshell: recent advances, J. Front. Bioeng. Biotechnol., 2021, 9. [Crossref], [Google Scholar], [Publisher]

[8] G. Ahlborn, B. W. Sheldon, Identifying the components in eggshell membrane responsible for reducing the heat resistance of bacterial pathogens, J. Food Prot., 2006, 69, 729-38. [Crossref], [Google Scholar], [Publisher]

[9] S.F. Bao, W. Windisch, M. Kirchgessner, Calcium bioavailability of different organic dietary source (citrate lactate, acetate, oyster-shell, eggshell, calcium phosphate), In J. Anim. Physiol. Anim. Nutr., 1997, 78, 154-160. [Crossref], [Google Scholar], [Publisher]

[10] H.C. Elliott, P.E. Mackie, R.A. Young, Monoclinic hydroxyapatite, Science, 1973, 180, 1055-1057. [Crossref], [Google Scholar], [Publisher]

[11] T.S.B. Nasaraju, D.E. Phebe, Some physico-chemical aspects of hydroxylapatite, J. Mat. Sci., 1966, 31, 1-21. [Crossref], [Google Scholar], [Publisher]

[12] W. Wang, K.W.K. Yeung, Bone grafts and Biomaterials Substitutes for Bone Defect Repair: a review, Bioact. Mater., 2017, 2, 224-247. [Crossref], [Google Scholar], [Publisher]

[13] T.T. Roberts, A.J. Rosenbaum, Bone grafts, bone substitutes and orthobiologics: the bridge between basic science and clinical advancements in fracture healing, Organogenesis, Oct-Dec., 2012, 8, 114-124. [Crossref], [Google Scholar], [Publisher]

[14] N.A.S. Mohd Pu'ad, J. Alipal, H.Z. Abdullah, M.I. Idris, T.C. Lee. Synthesis of Eggshell Derived hydroxyapatite via chemical precipitation and calcination method, Materials Today: Proceedings, 2021, 42, 172-177. [Crossref], [Google Scholar], [Publisher]

[15] N.D Malau, F. Adinugraha. Synthesis of hydrokxyapatite based duck egg shells using precipitation method, J. Phys.: Conf. Ser., 2020, 1563, 012020. [Crossref], [Google Scholar], [Publisher]

[16] Albuquerque, C.G. Monica, I. Jimenez-Urbistondo, J. Santamaria-Gonzalez, CaO supported on mesoporous silicas as basic catalysts for transesterification reactions, Appl Catal A: Gen, 2008, 334, 35-43. [Crossref], [Google Scholar], [Publisher]

[17] M.C. Reis, M.F.B. Sousa, F. Alobaid, C.A. Bertran, Y. Wang, A two-fluid model for calcium carbonate precipitation in highly supersaturated solutions, Adv. Powder Technol., 2018, 29, 1571-1581. [Crossref], [Google Scholar], [Publisher]

[18] P. Agrinier, A. Deutsch, U. Scharer, I. Martinez, Fast back-reactions of shock-released CO₂ from carbonates: an experimental approach, Geochim. Cosmochim. Acta., 2001, 65, 2615-2632. [Crossref], [Google Scholar], [Publisher]

[19] M.L. Granados, M.D. Poves, D.M. Alonso, R. Mariscal, F.C. Galisteo, T.R. Moreno, J. Santamaría, L.G. Fierro, Biodiesel from sunflower oil by using activated calcium oxide, Appl. Catal. B: Environ., 2007, 73, 317-326. [Crossref], [Google Scholar], [Publisher]

[20] R. Han, Y. Wang, S. Xing, C. Pang, Y. Hao, C. Song, Q. Liu, Progress in reducing calcination reaction temperature of calcium-looping CO₂ capture technology: a critical review, J. Chem. Eng., 2022, 450, 137952. [Crossref], [Google Scholar], [Publisher]

[21] K. Dahlan, F. Prasetyanti, Y.W. Sari, Synthesis of hydroxyapatite from eggshells using dry method, J. Biophysics, 2009, 5, 71-78. [Google Scholar]

[22] H. Pan, B.W. Darvell, Effect of carbonate on hydroxyapatite solubility, Crystal Growth & Amp; Design, 2010, 10, 845-850. [Crossref], [Google Scholar], [Publisher]

[23] N.A. Luchman, A.W.R. Megat, S.H.Z. Ariffin, N.S. Nasruddin, S.F. Lau, F.Yazid, Comparison between hydroxyapatite and polycaprolactone in inducing osteogenic differentiation and augmenting maxillary bone regeneration in rats, PeerJ., 2022, 10, e13356. [Crossref], [Google Scholar], [Publisher]

[24] M. Ozawa, S. Suzuki, Microstructural development of natural hydroxyapatite originated from fi sh-bone waste through heat treatment, J. Am. Ceram. Soc., 2002, 85, 1315-1317. [Crossref], [Google Scholar], [Publisher]

[25] R. Pallela, J. Venkatesan, S.K. Kim, Polymer assisted isolation of hydroxyapatite from Th unnus obesus bone, J. Ceram. Int., 2011, 37, 3489-3497. [Crossref], [Google Scholar], [Publisher]

[26] S. Kirubanandan, P.K. Sehgal, Regeneration of soft tissue using porous bovine collagen scaffold, J. Optoelectron. Biomed. Mate., 2010, 2. [Google Scholar], [PDF]

[27] X. Feng, Chemical and biochemical basis of cell-bone matrix interaction in health and disease, Curr Chem Biol., 2009, 3, 189-196. [Crossref], [Google Scholar], [Publisher]

[28] M.Z. Ichsan, Synthesis of collagen-hydroxyapatite composite macroporus as a bone graft candidate, Thesis, Department of Physics, Faculty of Science and Technology, Airlangga University: Surabaya., 2012 [Crossref]

Eurasian Chemical Communications

Fabrication and analysis of nano-hydroxyapatite [Ca10(PO4)6(OH)2] composites with collagen derived from eggshells through freeze-drying

References

References

Volume 5, Issue 12
December 2023
Pages 1096-1116

Fabrication and analysis of nano-hydroxyapatite [Ca10(PO4)6(OH)2] composites with collagen derived from eggshells through freeze-drying

References

References

Volume 5, Issue 12December 2023Pages 1096-1116

Volume 5, Issue 12
December 2023
Pages 1096-1116